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Biotechnology Making the world a better place!

Helps you identify immune cells efficiently and easily identify cell activation status

Good helper for scientific research, professional manufacturer

Wuhan Reivaa Biotech Co., Ltd.

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Hot Products

Development, production and sales of ELISA kits, antibodies, recombinant proteins and related reagents

Cell proliferation/toxicity/viability assay

Flow-through antibodies

Copper Death Detection Series

Mouse Micro Kit

TUNEL apoptosis assay

FCM Auxiliary Reagents

Glucolipid metabolism

One-Step (QuicKey Pro) Reagent Kit

Annexin V apoptosis assay

Fluorescein/Labeling Kit

Oxidative stress

Labeled Internal Reference Antibodies

Literature

Light regulates glucose metabolism via the retina-hypothalamus-brown adipose tissue axis

Production and development of vesicle-free cell culture additives for the preparation of human extracellular vesicles

Rod-shaped nanoparticle polymers interfere with neutrophils for effective treatment of ischemic stroke

HTRA activator plays a therapeutic role in papain-induced lung inflammation by limiting ILC activation

Online courseware Operation Guide FAQ Download

Intracellular target staining steps

Cell surface target staining steps

Preparation of single cell suspension from mouse spleen

Preparation of mouse bone marrow single cell suspension

QuicKey Pro ELISA competition method) operation video

QuicKey Pro ELISA (Dual Antibody Sandwich Method) Operation Video

Competition ELISA Video

Cell Metabolism Assay Experiment Frequently Asked Questions

How to calculate the results of metabolic tests

Iron Death Study - Cellular Ferrous Iron Assay FAQ

7 tips to teach you how to choose ELISA kits

Classification of HRP substrates

Are there any precautions for the pending markers?

The marker to be labeled needs to have a free primary amine or N-terminal, and the sample must not contain Tris, glycerol, proteins with amino groups or reagents.

Can this biotin labeling kit label other proteins besides antibodies?

The principle of the biotin labeling kit is that the ester bond of the activated biotin and the amino group on the labeled material are dehydrated and condensed to form a stable amide bond. In addition, the N-terminal end of the protein can also be labeled. If there is a primary amine on the protein, such as lysine is what can be labeled.

How can I tell if my sample needs to be diluted or if it needs other treatment?

The detection range of the kit is not equivalent to the concentration range of the sample to be tested. It is recommended to estimate the concentration of the sample to be tested from the relevant literature and to determine the actual concentration of the sample through pre-testing before the experiment. For routine sample types, consult ELISA technical support for recommended dilutions and schedule a pre-test. If the concentration of the analyte in the sample is too high or too low, dilute or concentrate the sample appropriately. If the analyte concentration in the sample is well below the minimum detection line of the kit, it is recommended to select a more sensitive kit for the assay.