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    Intracellular target staining steps


    Time:

    2023-05-16

    This video shows the procedure of Elabscience's intracellular target flow assay, demonstrating the steps of Fc receptor closure, surface target antibody incubation, fixation breaking, and intracellular target antibody incubation, providing experimentalists with guidance on the operation of intracellular target flow assay.

     

      Video description.

      This video shows the procedure of Elabscience's intracellular target flow assay, demonstrating the steps of Fc receptor closure, surface target antibody incubation, fixation breaking, and intracellular target antibody incubation, providing experimentalists with guidance on the operation of intracellular target flow assay.

      Experimental steps.

      Sample preparation→cell counting→control set→Fc receptor closure (optional)→surface indicator antibody deconjugation→fixed membrane breaking intracellular indicator antibody incubation→online detection

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