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    SARS-CoV-2 Nucleocapsid Protein IgG ELISA Kit


    The kit uses an indirect ELISA method. The recombinant purified SARS-CoV-2 Nucleocapsid protein (N protein) is pre-coated on the enzyme plate. The anti-SARS-CoV-2 Nucleocapsid protein IgG antibody in the sample or positive control will bind to the precoated SARS-CoV-2 Nucleocapsid protein antigen on the ELISA plate, and the free components will be washed away. Horseradish peroxidase-labeled mouse anti-human IgG secondary antibody is then added to form a SARS-CoV-2 nuclear coat protein antigen-anti-SARS-CoV-2 nuclear coat protein IgG-mouse anti-human IgG secondary antibody immune complex, and the free components are washed away. The chromogenic substrate (TMB) was added, and TMB appeared blue catalyzed by horseradish peroxidase and turned yellow after the addition of termination solution.

    SARS-CoV-2 Nucleocapsid Protein ELISA Kit


    The kit adopts the double antibody sandwich ELISA method. Anti-SARS-CoV-2 N protein antibody is used to coat the enzyme standard plate, and the sample (or standard) and biotinylated anti-SARS-CoV-2 N protein antibody are added to the enzyme standard plate at the same time during the experiment, the SARS-CoV-2 N protein in the sample (or standard) will bind to the coating antibody, while the anti-SARS-CoV-2 N protein antibody binds to the coating antibody with the SARS-CoV-2 N protein binds to the encapsulated antibody, and the free component is washed away.

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