SARS-CoV-2 Nucleocapsid Protein ELISA Kit

Item No:

E-EL-E604

Chinese Name:

Alias:

N protein, Protein N, NP, NC, Nucleoprotein, 2019-nCoV N protein

Keywords:

protein

Classification:

Recombinant protein

Retail Price

¥ 1400

Market Price

¥ 1400

Spec
- 48T
- 96T
- 96T*5

Quantity

Inventory surplus

2997

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Describe

Product Properties

Product specification	96T,96T*5
Experiment Length	3.5h
Sample type	Serum, plasma or other biological fluids
Sample volume	100 μL
Storage conditions	4°C/-20°C (store individual components according to instructions)
Test range	0.39-25 ng/mL
Product use	This kit is used for the in vitro quantification of N protein in serum, plasma or other biological fluids.
Repeatability	The intra-batch CV & inter-batch CV are less than 10%.
Product Alias	N protein, Protein N, NP, NC, Nucleoprotein, 2019-nCoV N protein

Principle of detection

　　The kit adopts the double antibody sandwich ELISA method. Anti-SARS-CoV-2 N protein antibody is used to coat the enzyme standard plate, and the sample (or standard) and biotinylated anti-SARS-CoV-2 N protein antibody are added to the enzyme standard plate at the same time during the experiment, the SARS-CoV-2 N protein in the sample (or standard) will bind to the coating antibody, while the anti-SARS-CoV-2 N protein antibody binds to the coating antibody with the SARS-CoV-2 N protein binds to the encapsulated antibody, and the free component is washed away. The horseradish peroxidase-labeled affin was added, and the biotin specifically bound to the affin to form an immune complex, and the free component was washed away. The chromogenic substrate (TMB) was added, and the TMB showed blue color catalyzed by horseradish peroxidase and turned yellow after the addition of termination solution. The concentration of SARS-CoV-2 N protein in the sample was calculated by plotting the standard curve.

Operation steps

	1. Add 100 μL of standard working solution or sample to the corresponding plate wells and incubate at 37°C for 90 minutes
	2. Immediately after discarding the liquid in the plate, add 100 μL of biotinylated antibody working solution and incubate at 37°C for 60 minutes
	3. Discard the liquid in the plate and wash the plate 3 times
	4. Add 100μL of HRP enzyme conjugate working solution to each well, incubate at 37℃ for 30 minutes, discard the liquid in the plate, and wash the plate 5 times
	5. Add 90μL of substrate solution to each well and incubate at 37℃ for 15 minutes
	6. Add 50μL of termination solution to each well
	7. Read immediately at 450 nm and process the data