SARS-CoV-2 Nucleocapsid Protein IgG ELISA Kit

Item No:

E-EL-E600

Chinese Name:

Alias:

COVID 19 IgG,N protein IgG

Keywords:

Multi-resistance

protein

Classification:

Multi-resistance

Retail Price

¥ 2400

Market Price

¥ 1

The kit uses an indirect ELISA method. The recombinant purified SARS-CoV-2 Nucleocapsid protein (N protein) is pre-coated on the enzyme plate. The anti-SARS-CoV-2 Nucleocapsid protein IgG antibody in the sample or positive control will bind to the precoated SARS-CoV-2 Nucleocapsid protein antigen on the ELISA plate, and the free components will be washed away. Horseradish peroxidase-labeled mouse anti-human IgG secondary antibody is then added to form a SARS-CoV-2 nuclear coat protein antigen-anti-SARS-CoV-2 nuclear coat protein IgG-mouse anti-human IgG secondary antibody immune complex, and the free components are washed away. The chromogenic substrate (TMB) was added, and TMB appeared blue catalyzed by horseradish peroxidase and turned yellow after the addition of termination solution.

Spec
- 48T
- 96T
- 96T*5

Quantity

Inventory surplus

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Describe

Product Properties

Product specification	96T,96T*5
Experiment Length	1.5h
Sample type	Human serum, plasma
Sample volume	100 μL
Storage conditions	2-8℃
Test range	Qualitative Assay
Product use	This kit is used for the in vitro qualitative detection of SARS-CoV-2 IgG in human serum and plasma.
Repeatability	Both intra-batch CV & inter-batch CV are less than 10%.
Product alias	COVID 19 IgG,N protein IgG
Experiment type	Indirect-ELISA

Detection principle

Operation steps

	1. Add 100 μL of the sample and the quality control to the corresponding plate wells and incubate at 37°C for 45 minutes.
	2. Discard the liquid in the plate and wash the plate 3 times.
	3. Add 100uL of binding solution to each well and incubate at 37°C for 30 minutes. Discard the liquid in the plate and wash the plate 5 times.
	4. Add 90 μL of the conjugate solution to each well and incubate at 37C for about 15 minutes.
	5. . Add 50μL of stop solution to each well
	6. Read immediately at 450nm and process the data