2023-05-30
Nature: PI3Kα small molecule activator for cardioprotection and neuroregeneration
The PI3K family consists of three classes of isozymes, of which type IA (class IA PI3K) has been most extensively and intensively studied and consists of PI3K p110 catalytic subunits (PI3Kα, PI3Kβ and PI3Kδ) and PI3K p85 regulatory subunits with lipid kinase activity and protein kinase activity. type IA PI3K and its effectors AKT and mTORC1 are overactivated in cancer and the immune system, which has driven the widespread development of PI3K pathway inhibitors.
2023-05-30
Role of Ninj2 gene in the development of the nervous system
Ninj2基因是一个编码神经元特异性蛋白的基因,其在神经系统发育中发挥着重要作用。本研究通过对Ninj2基因进行敲除实验,发现敲除Ninj2基因会导致小鼠神经系统发育异常,表现为神经元数量减少、突触形成异常和行为学缺陷等。此外,我们还发现Ninj2基因可以通过调节Notch信号通路来影响神经系统发育。这些结果表明Ninj2基因在神经系统发育中具有重要的作用,为深入研究神经系统发育提供了新的思路和方向。
2023-05-16
Cell Metabolism Assay Experiment Frequently Asked Questions
The culture medium was aspirated and the cells were gently washed over with cold PBS (0.01 M, pH 7.4), then the cells were collected by trypsin digestion or cell scraping, 2-5 mL of PBS (0.01 M, pH 7.4) was added to make a cell suspension, and the cells were collected by centrifugation at 4°C, 1000 × g for 5 min.
2023-05-16
How to calculate the results of metabolic tests
Metabolic testing is a method that uses chemical reactions to detect various products and enzymes in the biological metabolic process. Its main detection targets include substances such as sugars, lipids, organic small molecules, inorganic ions, and proteins, as well as various types of enzymes involved in the metabolic reaction process. The assay is usually performed using a colorimetric method (measuring absorbance) or a fluorometric method (measuring fluorescence intensity).
2023-05-16
Iron Death Study - Cellular Ferrous Iron Assay FAQ
Increased cellular ferrous content is a key feature of iron death that distinguishes it from other forms of cell death, and its detection is one of the most common indicators of iron death research. Based on the hot market feedback, Elabscience® has increased its R&D and optimization efforts and launched a highly sensitive ferrous ion colorimetric test kit specifically for cellular samples
2023-05-16
7 tips to teach you how to choose ELISA kits
There are various ELISA kits on the market, with varying quality. How to choose the best ELISA kits that meet the needs of research?
2023-05-16
Classification of HRP substrates
The substrate range is wide and includes diaminobenzidine (DAB), chloronaphthol and aminomethylcarbazole.
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