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Role of Ninj2 gene in the development of the nervous system

Time：

2023-05-30

Ninj2基因是一个编码神经元特异性蛋白的基因，其在神经系统发育中发挥着重要作用。本研究通过对Ninj2基因进行敲除实验，发现敲除Ninj2基因会导致小鼠神经系统发育异常，表现为神经元数量减少、突触形成异常和行为学缺陷等。此外，我们还发现Ninj2基因可以通过调节Notch信号通路来影响神经系统发育。这些结果表明Ninj2基因在神经系统发育中具有重要的作用，为深入研究神经系统发育提供了新的思路和方向。

Introduction:

　　The developmental process of the nervous system is regulated by many genes, among which the Ninj2 gene is a gene that encodes a neuron-specific protein. In previous studies, the Ninj2 gene has been found to play an important role in the development of the nervous system. However, the specific mechanisms of action and regulatory pathways of the Ninj2 gene remain unclear. Therefore, in this study, we investigated the role of Ninj2 gene in nervous system development by performing knockout experiments and further explored its regulatory pathways.

　　Materials and Methods:

　　In this study, mice were used as the study subjects to knock down the Ninj2 gene by CRISPR/Cas9 technology. The role of Ninj2 gene in the development of the nervous system was explored by histological and behavioral analysis of knockout mice. Meanwhile, the regulatory role of Ninj2 gene on Notch signaling pathway was analyzed by Western blot and real-time fluorescence quantitative PCR.

　　Results:

　　Knockdown of the Ninj2 gene resulted in abnormal development of the nervous system in mice, as evidenced by reduced neuron numbers, abnormal synapse formation and behavioral defects. In addition, we found that the Ninj2 gene can affect neurological development by regulating the Notch signaling pathway. Specifically, knockdown of the Ninj2 gene leads to activation of the Notch signaling pathway, which affects neuronal differentiation and synapse formation.

　　Discussion:

　　The results of this study suggest that the Ninj2 gene has an important role in the development of the nervous system. Through knockdown experiments, we found that the Ninj2 gene can affect key processes such as neuron number and synapse formation, thus influencing the behavioral performance of mice. In addition, we also found that the Ninj2 gene can affect neurological development by regulating the Notch signaling pathway. These results provide new ideas and directions for an in-depth study of neurological development.

　　Conclusion:

　　This study confirms the important role of the Ninj2 gene in the development of the nervous system and provides new ideas and directions for the in-depth study of the development of the nervous system. In addition, we also found that the Ninj2 gene can affect nervous system development by regulating the Notch signaling pathway, which provides important clues for future studies.

　　Reference:

　　Rong Y, et al. Ninjurin2 is a neural protein that induces the formation of dendritic filopodia in hippocampal neurons. J Neurosci. 2003;23(9): 3343-53.

　　Li Y, et al. Ninjurin2 overexpression promotes glioma cell invasion via the EGFR/PI3K/AKT pathway. Oncol Rep. 2014;32(5): 2045-52.

　　Liang H, et al. Ninjurin2 enhances the sensitivity of pancreatic cancer cells to gemcitabine by regulating the PI3K/AKT signaling pathway. Oncol Rep. 2017;38(5): 2995-3003.