Our products are for in vitro research use only, not for clinical diagnosis

Nickname | quit

Recently added:

in total 0 items Total 0

Language:

Home
Product
Research
Services
News
About

All

All
Products
News
Introduction
FAQ
Video
Gallery

All

All
Products
News
Introduction
FAQ
Video
Gallery

Home page

Collection

Contrast

No .

E-EL-R0012c

Rat IL-1β(Interleukin 1 Beta) ELISA Kit

Item No:

E-EL-R0012c

Chinese Name:

Alias:

IL1B, IL1-BETA, IL1F2, catabolin

Keywords:

IHC kits

Classification:

IHC kits

Retail Price

¥ 2400

Market Price

¥ 2400

This kit is used for the in vitro quantification of IL-1β in Rat serum, plasma or other biological fluids

Spec
- 48T
- 96T
- 96T*5

Quantity

Inventory surplus

2997

隐藏域元素占位

Product Comparison

Total X Clear all

Describe

Use

　　This kit is used for the in vitro quantification of IL-1β in Rat serum, plasma or other biological fluids

Detection principle

　　This kit adopts double antibody sandwich ELISA method. Anti-Rat IL-1β antibody is used to coat the ELISA plate. The rat IL-1β in the sample or standard will bind to the coating antibody during the experiment, and the free components are washed away. Biotinylated anti-rat IL-1β antibody and horseradish peroxidase-labeled affin were added sequentially. The anti-rat IL-1β antibody binds to the rat IL-1β bound to the encapsulated antibody, and the biotin binds specifically to the affin to form an immune complex, and the free components are washed away. The chromogenic substrate (TMB) was added, and TMB appeared blue catalyzed by horseradish peroxidase and turned yellow after the addition of termination solution. The concentration of IL-1β in the sample was calculated by plotting the standard curve, using an enzyme marker to measure the OD value at 450 nm, with a positive ratio between IL-1β concentration and OD450 value.

Reaction Type	Sandwich method
Specification	96T
Reaction time	3.5h
Reactivity	Rat
Detection method	Colormetric
Detection range	31.25—2000 pg/mL
Sensitivity	18.75 pg/mL
Sample volume	100μL
Sample type	Serum, plasma or other biological fluids

Specificity

　　Detects rat IL-1β in samples with no significant cross-reactivity with other related proteins.

Repeatability

　　Within the plates, the coefficients of variation between the plates were <10%.

Typical Data

　　The OD values of the standard curves may vary due to different experimental operating conditions (e.g. operator, pipetting technique, plate washing technique and stabilization conditions, etc.). The following data and curves are for reference only. Experimenters need to establish standard curves according to their own experiments.

(pg/mL)	O.D	Average	Corrected
2000	2.388 2.442	2.415	2.349
1000	1.602 1.622	1.612	1.546
500	0.956 0.941	0.949	0.883
250	0.494 0.53	0.512	0.446
125	0.266 0.242	0.254	0.188
62.5	0.169 0.163	0.166	0.1
31.25	0.114 0.122	0.118	0.052
0	0.06 0.071	0.066	--

Precision

　　 Intra-plate precision: 20 times on 1 plate for low, medium and high concentration samples.
　　 Intra-plate precision: 20 times on 3 plates for low, medium and high concentrations.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	100.50	314.20	880.10	107.40	340.60	954.80
Standard deviation	5.70	13.50	30.80	5.60	20.40	33.40
C V (%)	5.67	4.30	3.50	5.21	5.99	3.50

Recovery rate

　　Recovery experiments were performed by adding known concentrations of the target protein to five different samples, and the recovery range and average recovery were obtained.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=8)	85-98	91
EDTA plasma (n=8)	86-100	93
Cell culture media (n=8)	86-101	92

Linearity

　　Five samples were spiked with known concentrations of target proteins, and the recovery experiments were performed to obtain the range of recovery rates and average recovery rates. Five samples were diluted 2, 4, 8 and 16 times for recovery experiments, and the range of recovery rates and average recovery rates were obtained.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	86-100	86-96	100-116
1:2	Average (%)	92	91	106
1:4	Range (%)	95-105	84-96	94-106
1:4	Average (%)	100	91	100
1:8	Range (%)	101-114	81-91	96-112
1:8	Average (%)	108	86	103
1:16	Range (%)	96-108	83-97	92-106
1:16	Average (%)	102	89	99

Reagent kit composition and storage

　　Unopened kits can be stored at 2-8°C for one week; if the kit is used after one week, please unpack the kit and store each component separately according to the conditions in the table below.

Chinese name	English Name	Specification	Storage conditions
ELISA enzyme standard plate (detachable)	Micro ELISA Plate(Dismountable)	8 holes×12 strips	-20℃, can be stored for 6 months
Lyophilized standards	Reference Standard	2 sticks
Concentrated biotinylated antibody(100×)	Concentrated Biotinylated Detection Ab	1 x 120 μL
Concentrated HRP enzyme conjugate(100×)	Concentrated HRP Conjugate	1 x 120 μL	-20℃(keep away from light), can be stored for 6 months
Standard & Sample Diluent	Reference Standard & Sample Diluent	1 bottle of 20 mL	2-8℃. Can be stored for 6 months
Biotinylated antibody diluent	Biotinylated Detection Ab Diluent	1 bottle 14 mL
Enzyme Conjugate Diluent	HRP Conjugate Diluent	1 bottle 14 mL
Concentrated Washing Solution(25×)	Concentrated Wash Buffer (25×)	1 bottle 30 mL
Substrate solution (TMB)	Substrate Reagent	1 bottle 10 mL	2-8℃(keep away from light)
Reaction termination solution	Stop Solution	1 bottle 10 mL	2-8℃
Plate lamination	Plate Sealer	5 sheets
Product manual	Product Description	1 copy
Quality inspection report	Certificate of Analysis	1 copy

　　Note: All reagent bottles must be tightly capped to prevent evaporation and microbial contamination.
　　The volume of reagents is subject to the actual shipping instructions. The relevant reagents will be slightly more than the volume indicated on the label when dispensing, please measure rather than pour directly when using.

Self-provided items required for the test

1. ELISA (450 nm wavelength filter)

2. High-precision pipettes, EP tubes and disposable tips: 0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL

3. 37°C thermostat, double-distilled water or deionized water

4. absorbent paper

操作步骤

	1. Add 100 μL of standard working solution or sample to the corresponding plate wells and incubate at 37°C for 90 minutes
	2. After discarding the liquid in the plate, add 100 μL of biotinylated antibody working solution and incubate at 37°C for 60 minutes.
	3. Discard the liquid in the plate and wash the plate 3 times
	4. Add 100μL of HRP enzyme conjugate working solution to each well, incubate at 37℃ for 30 minutes, discard the liquid in the plate, and wash the plate 5 times
	5. Add 90μL of substrate solution to each well and incubate at 37℃ for 15 minutes
	6. Add 50μL of termination solution to each well
	7. Read immediately at 450 nm and process the data