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    How does the "repeated freeze-thaw" process mentioned in tissue/cell sample collection work?


    Tissue samples: After tissue grinding, place them at -80°C for 1h/liquid nitrogen for 0.5h, and then place them in a 30°C water bath with slight shaking to make them thaw rapidly, and this operation can be repeated 1-2 times.
    Cell samples: Repeat the above operation of freezing and thawing 2-3 times. In case of membrane proteins, it can be sonicated properly, but the temperature and frequency of sonication need to be controlled.
    It is recommended to add protease inhibitors to the sample in advance. Routine recommendation: PMSF.

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